Electrophoresis

Genes, which are short DNA strands can be detected and compared through a DNA electrophoresis. DNA electrophoresis uses a high voltage DC power supply, like the Revolutionary Science RS-PS-75 and a DNA gel electrophoresis chamber, called the DNA RevBox. An agarose gel is made by mixing agarose powder and distilled water and boiling it in a microwave or Mini Pro Bath. When liquefied and homogeneous, the agarose can be poured into the casting tray, with a comb to create small wells where the DNA, mixed with electrophoresis dye can be inserted. When the gel and buffer solution and dyed DNA samples are added to the gel box, a DC current is applied using the power supply. As electrons move across the gel from the negative terminal (anode) to the positive terminal (cathode), the dyed DNA genes will advance across the gel based on size and charge. Genes that are smaller move faster across the gel, while genes that are positively charge will move in the opposite direction as the genes that are negatively charged.

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